Short answer
For a 5mg peptide vial, concentration depends on diluent volume. With 1 mL it is 5 mg/mL, with 2 mL it is 2.5 mg/mL, and with 5 mL it is 1 mg/mL.
Step-by-step
- Start with the vial amount: 5 mg.
- Choose the diluent volume from the research protocol.
- Divide 5 mg by the diluent volume in mL.
- Use the resulting mg/mL value as the same numeric value in mcg/uL.
5mg peptide reconstitution formula
The formula is 5 mg / diluent volume in mL = concentration in mg/mL. If 5 mg is mixed with 2 mL, the result is 2.5 mg/mL, which is also 2.5 mcg/uL.
How to calculate amount from the 5mg chart
After concentration is known, multiply mcg/uL by the selected volume in uL to calculate total micrograms. For example, 2.5 mcg/uL multiplied by 40 uL equals 100 mcg.
What this chart does not do
This chart does not recommend a dose, diluent, protocol, schedule, route, or use case. It only shows arithmetic relationships for research planning and unit checking.
| Diluent volume | Concentration | Amount in 20 uL | Amount in 40 uL |
|---|---|---|---|
| 1 mL | 5 mg/mL = 5 mcg/uL | 100 mcg | 200 mcg |
| 2 mL | 2.5 mg/mL = 2.5 mcg/uL | 50 mcg | 100 mcg |
| 2.5 mL | 2 mg/mL = 2 mcg/uL | 40 mcg | 80 mcg |
| 5 mL | 1 mg/mL = 1 mcg/uL | 20 mcg | 40 mcg |