Short answer
Reconstitution math is mass divided by volume. For example, 5 mg of peptide reconstituted with 2 mL of diluent creates a 2.5 mg/mL solution.
Step-by-step
- Confirm peptide mass from a reliable label or certificate of analysis.
- Confirm the diluent and volume from the relevant research protocol.
- Divide mass in mg by volume in mL to calculate mg/mL.
- Use the concentration to calculate aliquot volumes only after checking the formula.