Short answer
Reconstitution charts use one formula: peptide mass divided by final volume. A 5 mg vial with 2 mL final volume is 2.5 mg/mL, which is also 2.5 mcg/uL.
Reference chart
Peptide reconstitution chart.
A compact reference for checking concentration and microliter volume relationships. Use it to audit math, not to choose a protocol.
How to read it
Use the chart as an audit, not a protocol.
- Find the row with the matching peptide mass and final volume.
- Read concentration as mg/mL and the same number as mcg/uL.
- Multiply mcg/uL by uL to check the amount in a selected volume.
- Use the calculator when your exact values are not shown.
Formula
Three lines explain every value below
concentration = mass / final volume
mg/mL = mcg/uL numerically
amount = mcg/uL x uL
mg/mL = mcg/uL numerically
amount = mcg/uL x uL
Concentration chart
Common mass and final volume examples
The last two columns show total amount in 20 uL and 40 uL after concentration is known.
| Mass | Final volume | mg/mL | mcg/uL | 20 uL | 40 uL |
|---|---|---|---|---|---|
| 2 mg | 1 mL | 2 mg/mL | 2 mcg/uL | 40 mcg | 80 mcg |
| 2 mg | 2 mL | 1 mg/mL | 1 mcg/uL | 20 mcg | 40 mcg |
| 5 mg | 1 mL | 5 mg/mL | 5 mcg/uL | 100 mcg | 200 mcg |
| 5 mg | 2 mL | 2.5 mg/mL | 2.5 mcg/uL | 50 mcg | 100 mcg |
| 5 mg | 2.5 mL | 2 mg/mL | 2 mcg/uL | 40 mcg | 80 mcg |
| 10 mg | 2 mL | 5 mg/mL | 5 mcg/uL | 100 mcg | 200 mcg |
| 10 mg | 5 mL | 2 mg/mL | 2 mcg/uL | 40 mcg | 80 mcg |
Target-volume table
Volume needed for common target amounts
This table starts with known concentration. It does not choose a target amount.
| Concentration | 25 mcg | 50 mcg | 100 mcg | 250 mcg |
|---|---|---|---|---|
| 1 mcg/uL | 25 uL | 50 uL | 100 uL | 250 uL |
| 2 mcg/uL | 12.5 uL | 25 uL | 50 uL | 125 uL |
| 2.5 mcg/uL | 10 uL | 20 uL | 40 uL | 100 uL |
| 5 mcg/uL | 5 uL | 10 uL | 20 uL | 50 uL |
| 10 mcg/uL | 2.5 uL | 5 uL | 10 uL | 25 uL |
Worked example
5 mg in 2 mL
Concentration: 5 mg / 2 mL = 2.5 mg/mL.
Equivalent: 2.5 mg/mL = 2.5 mcg/uL.
Volume check: 100 mcg / 2.5 mcg/uL = 40 uL.
Check a calculator result
Find the nearest mass and final volume pair, then confirm that the concentration matches the calculator output.
Avoid unit drift
Read mg/mL as the same numeric value in mcg/uL before calculating amount in a microliter volume.
Know when to stop
If your exact mass, volume, or target amount is not shown, use the calculator instead of rounding from the chart.
Calculator
Check exact values
Charts are quick references. For exact mass, final volume, or target amount values, use the calculator.
Reconstitution result
2.5 mg/mL2.5 mg/mL equals 2.5 mcg/uL.
Read this as concentration first, then volume second. The calculator is not checking whether the source material or workflow is appropriate.
- Total peptide
- 5,000 mcg
- Aliquot volume
- 40 uL
- Approx. aliquots
- 50
- Formula
- 5 mg / 2 mL
Boundaries
What the chart cannot verify
The chart cannot verify product identity, label accuracy, certificate of analysis, sterility, storage, diluent suitability, administration route, schedule, legality, clinical use, or safety. It only shows arithmetic relationships.
NIST SI prefix guidance ->FAQ
Reconstitution chart questions
How do you use a peptide reconstitution chart?
Find the row with the matching peptide mass and final volume, then read the concentration as mg/mL and the same numeric value as mcg/uL.
What formula is used in a reconstitution chart?
The main formula is concentration = peptide mass / final volume. Amount in a selected volume is concentration in mcg/uL multiplied by volume in uL.
Can a reconstitution chart choose how much diluent to use?
No. A chart only shows arithmetic relationships. Diluent choice, final volume, and workflow decisions must come from validated research documentation or qualified professional guidance.
When should you use the calculator instead of the chart?
Use the calculator when your exact peptide mass, final volume, or target amount is not shown in the chart.
Is this guide medical advice?
No. CalcPeptides guides explain arithmetic and terminology for education and research planning only.
Should calculator results be independently checked?
Yes. Always verify calculator results against validated protocols, labels, certificates of analysis, and qualified professional review.
What do uL, µL, mcg, and µg mean?
uL and µL both mean microliter. mcg and µg both mean microgram. CalcPeptides uses uL and mcg because they are easier to type.