Reference chart

Peptide reconstitution chart.

Concentration equals mass divided by final solution volume. Build a chart for your values, or browse the fixed examples below. Print it, save a PDF, or download a CSV for your reference notes.

Build your chart Use calculator

Your reference chart

Use your own mass and volume

Enter the final volume after mixing. Each row shows the amount in a volume you select. The default rows are arithmetic examples.

CalcPeptides reference chart

5 mg in 2 mL final volume

Concentration: 2.5 mg/mL = 2.5 mcg/uL

Amount (mcg) = concentration (mcg/uL) x volume (uL). Assumes a uniform solution; losses are excluded.

Arithmetic reviewed October 5, 2026. uL means microliter; mcg means microgram.

Show mL and mg columns

Amount contained in each selected solution volume
Volume (uL)	Volume (mL)	Amount (mcg)	Amount (mg)	Calculator
10	0.01	25	0.025	Check row
20	0.02	50	0.05	Check row
40	0.04	100	0.1	Check row
100	0.1	250	0.25	Check row
200	0.2	500	0.5	Check row

Reference calculations only. The chart does not choose a treatment amount or validate material suitability. Source: https://calcpeptides.com/guides/peptide-reconstitution-chart/

Convert other units

Print includes all units and the formula. Choose Save as PDF in your browser's print dialog. CSV includes the inputs, units, formula, assumptions, and review date.

View or copy CSV

If downloads are unavailable, copy this text into a .csv file or a spreadsheet import. It contains the same inputs, units, formula, assumptions, and source as the download. Values stay in this browser.

CSV reference data

mass_mg,final_volume_mL,concentration_mg_per_mL,volume_uL,volume_mL,amount_mcg,amount_mg,formula,assumption,arithmetic_reviewed,source
5,2,2.5,10,0.01,25,0.025,amount_mcg = concentration_mcg_per_uL * volume_uL,Uniform solution; final volume after mixing; losses excluded,2026-10-05,https://calcpeptides.com/guides/peptide-reconstitution-chart/
5,2,2.5,20,0.02,50,0.05,amount_mcg = concentration_mcg_per_uL * volume_uL,Uniform solution; final volume after mixing; losses excluded,2026-10-05,https://calcpeptides.com/guides/peptide-reconstitution-chart/
5,2,2.5,40,0.04,100,0.1,amount_mcg = concentration_mcg_per_uL * volume_uL,Uniform solution; final volume after mixing; losses excluded,2026-10-05,https://calcpeptides.com/guides/peptide-reconstitution-chart/
5,2,2.5,100,0.1,250,0.25,amount_mcg = concentration_mcg_per_uL * volume_uL,Uniform solution; final volume after mixing; losses excluded,2026-10-05,https://calcpeptides.com/guides/peptide-reconstitution-chart/
5,2,2.5,200,0.2,500,0.5,amount_mcg = concentration_mcg_per_uL * volume_uL,Uniform solution; final volume after mixing; losses excluded,2026-10-05,https://calcpeptides.com/guides/peptide-reconstitution-chart/

How to read it

Use the chart as an audit, not a protocol.

Find the peptide mass row that matches the vial.
Match the final solution volume after mixing, rather than just the volume added.
Read the resulting concentration as mg/mL and mcg/uL.
Multiply concentration in mcg/uL by a selected volume in uL to check the amount in that volume.

Formula

Three lines explain every value below

concentration = mass / final volume
mg/mL = mcg/uL numerically
amount = mcg/uL x uL

Concentration chart

Common mass and final volume examples

The last two columns show total amount in 20 uL and 40 uL after concentration is known.

Scroll the table sideways to see all columns.

Mass	Final volume	mg/mL	mcg/uL	20 uL	40 uL
2 mg	1 mL	2 mg/mL	2 mcg/uL	40 mcg	80 mcg
2 mg	2 mL	1 mg/mL	1 mcg/uL	20 mcg	40 mcg
5 mg	1 mL	5 mg/mL	5 mcg/uL	100 mcg	200 mcg
5 mg	2 mL	2.5 mg/mL	2.5 mcg/uL	50 mcg	100 mcg
5 mg	2.5 mL	2 mg/mL	2 mcg/uL	40 mcg	80 mcg
10 mg	2 mL	5 mg/mL	5 mcg/uL	100 mcg	200 mcg
10 mg	5 mL	2 mg/mL	2 mcg/uL	40 mcg	80 mcg

Target-volume table

Volume needed for common target amounts

This table starts with known concentration. It does not choose a target amount.

Scroll the table sideways to see all columns.

Concentration	25 mcg	50 mcg	100 mcg	250 mcg
1 mcg/uL	25 uL	50 uL	100 uL	250 uL
2 mcg/uL	12.5 uL	25 uL	50 uL	125 uL
2.5 mcg/uL	10 uL	20 uL	40 uL	100 uL
5 mcg/uL	5 uL	10 uL	20 uL	50 uL
10 mcg/uL	2.5 uL	5 uL	10 uL	25 uL

Worked example

5 mg in 2 mL

Concentration: 5 mg / 2 mL = 2.5 mg/mL.

Equivalent: 2.5 mg/mL = 2.5 mcg/uL.

Volume check: 100 mcg / 2.5 mcg/uL = 40 uL.

Check a calculator result

Find the nearest mass and final volume pair, then confirm that the concentration matches the calculator output.

Avoid unit drift

Read mg/mL as the same numeric value in mcg/uL before calculating amount in a microliter volume.

Know when to stop

Enter your exact mass and final volume in the custom chart. For a target amount, use the calculator instead of rounding from an example.

Calculator

Check exact values

To find the volume for an amount already specified in your research documentation, use the reconstitution calculator. Use the converter to switch between mg, mcg, mL, and uL.

Reconstitution calculator Unit converter Concentration explained

Boundaries

What the chart cannot verify

The chart cannot verify product identity, label accuracy, certificate of analysis, sterility, storage, diluent suitability, administration route, schedule, legality, clinical use, or safety. It only shows arithmetic relationships.

NIST SI prefix guidance ->

FAQ

Reconstitution chart questions

How do you use a peptide reconstitution chart?

Find the row with the matching peptide mass and final volume, then read the concentration as mg/mL and the same numeric value as mcg/uL.

What formula is used in a reconstitution chart?

The main formula is concentration = peptide mass / final volume. Amount in a selected volume is concentration in mcg/uL multiplied by volume in uL.

Can a reconstitution chart choose how much diluent to use?

No. A chart only shows arithmetic relationships. Diluent choice, final volume, and workflow decisions must come from validated research documentation or qualified professional guidance.

When should you use the calculator instead of the chart?

Use the calculator when your exact peptide mass, final volume, or target amount is not shown in the chart.

Is this guide medical advice?

No. CalcPeptides guides explain arithmetic and terminology for education and research planning only.

Should calculator results be independently checked?

Yes. Always verify calculator results against validated protocols, labels, certificates of analysis, and qualified professional review.

What do uL, µL, mcg, and µg mean?

uL and µL both mean microliter. mcg and µg both mean microgram. CalcPeptides uses uL and mcg because they are easier to type.